Anti-APAF1 antibody
| Name | Anti-APAF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2000 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide, corresponding to amino acids 1158-1177 of Human Apaf 1 |
| Description | Rabbit Polyclonal |
| Gene | APAF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab2000 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2000, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/mlLane 1 : Human heart tissue lysate with absence of blocking peptideLane 2 : Human heart tissue lysate with presence of blocking peptide
Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.