Anti-Apc2 antibody [1A6]

• Supplier: Abcam
• Catalog: ab123855
• Prices: $370.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 1A6
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Monkey
• Antigen: Full length Human recombinant Apc2 protein produced in HEK293 cell (NP_037498)
• Description: Mouse Monoclonal
• Gene: ANAPC2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Apc2 antibody [1A6] (ab123855) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY Apc2 cDNALysates/proteins at 5 µg per lane.

All lanes : Anti-Apc2 antibody [1A6] (ab123855) at 1/200 dilutionLane 1 : Extracts from HepG2 cell lineLane 2 : Extracts from HeLa cell lineLane 3 : Extracts from SVT2 cell lineLane 4 : Extracts from A549 cell lineLane 5 : Extracts from COS7 cell lineLane 6 : Extracts from Jurkat cell lineLane 7 : Extracts from MDCK cell lineLane 8 : Extracts from PC12 cell lineLane 9 : Extracts from MCF7 cell lineLysates/proteins at 35 µg per lane.

ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded adenocarcinoma of Human breast tissue by Immunohistochemistry.

ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded adenocarcinoma of Human endometrium tissue by Immunohistochemistry.

ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded carcinoma of Human bladder tissue by Immunohistochemistry.

ab123855, at 1/150 dilution, staining Apc2 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.

ab123855 staining Apc2 in mouse mammary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with the primary antibody (1/150) for 20 hours at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.See Abreview

ab123855, at 1/100 dilution, staining Apc2 in COS7 cells transiently transfected by pCMV6-ENTRY Apc2 by Immunofluorescence.

ab123855, at 1/100 dilution staining Apc2 in HEK293T cells transfected with either pCMV6-ENTRY Apc2 overexpress plasmid(Red) or empty vector control plasmid (Blue) by flow cytometry.