Anti-Apg7 antibody [EP1759Y]

• Supplier: Abcam
• Catalog: ab52472
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1759Y
• Applications: ICC/IF ICC/IF WB IP ICC/IF FC IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues near the C terminus of human Apg7
• Description: Rabbit Monoclonal
• Gene: ATG7
• Conjugate: Unconjugated

Product images

Anti-Apg7 antibody [EP1759Y] (ab52472) at 1/500000 dilution + Jurkat cell lysate at 10 µgSecondarygoat anti-rabbit HRP labeled at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded human liver using ab52472 at a 1/100 dilution.

ICC/IF image of ab52472 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52472, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HEK293 cells stained with ab52472 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Product References

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