Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1XPBS (negative control) using ab178701, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized Jurkat cells labeling APOBEC3G with ab178701 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunofluorescent analysis of A375 cells labeling APOBEC3G with ab178701 at 1/100 dilution (green). DAPI nuclear staining (blue).
All lanes : Anti-APOBEC3G antibody [EPR11660] (ab178701) at 1/10000 dilutionLane 1 : A375 cell lysateLane 2 : IM-9 cell lysateLane 3 : HL60 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.