Anti-Apolipoprotein A I antibody [12C8]
| Name | Anti-Apolipoprotein A I antibody [12C8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab17278 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 12C8 |
| Applications | WB IHC-P ELISA |
| Species Reactivities | Human |
| Antigen | Full length native Apolipoprotein A1, isolated from human plasma |
| Description | Mouse Monoclonal |
| Gene | APOA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Apolipoprotein A I antibody [12C8] (ab17278) at 5 µg/mlLane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 2 : Lung (Human) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryLane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionLane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8408_Apolipoprotein-A-I-Primary-antibodies-ab17278-2.jpg)
All lanes : Anti-Apolipoprotein A I antibody [12C8] (ab17278) at 5 µg/mlLane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 2 : Lung (Human) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryLane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionLane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Ab17278 staining human normal skeletal muscle. Staining is localised to the cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism - Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism
Johnson LA, Kim HS, Knudson MJ, Nipp CT, Yi X, Maeda N. J Lipid Res. 2013 Feb;54(2):386-96.
Inhibition of cholesteryl ester transfer protein by torcetrapib modestly - Inhibition of cholesteryl ester transfer protein by torcetrapib modestly
Yvan-Charvet L, Matsuura F, Wang N, Bamberger MJ, Nguyen T, Rinninger F, Jiang XC, Shear CL, Tall AR. Arterioscler Thromb Vasc Biol. 2007 May;27(5):1132-8. Epub 2007 Feb 22.
HDL from CETP-deficient subjects shows enhanced ability to promote cholesterol - HDL from CETP-deficient subjects shows enhanced ability to promote cholesterol
Matsuura F, Wang N, Chen W, Jiang XC, Tall AR. J Clin Invest. 2006 May;116(5):1435-42.