Anti-Apolipoprotein CIII antibody
| Name | Anti-Apolipoprotein CIII antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21032 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human, Monkey |
| Antigen | Full length protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | APOC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab21032 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/mlLane 1 : Human Apolipoprotein CIII full length protein (ab77944) at 0.1 µgLane 2 : Human Apolipoprotein CIII full length protein (ab77944) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21032 as capture antibody. Anti-human Apolipoprotein CIII polyclonal antibody (ab21032 at 10 µg/ml) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.
Product References
Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc - Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc
Schjoldager KT, Vakhrushev SY, Kong Y, Steentoft C, Nudelman AS, Pedersen NB, Wandall HH, Mandel U, Bennett EP, Levery SB, Clausen H. Proc Natl Acad Sci U S A. 2012 Jun 19;109(25):9893-8. doi:
Identification and quantification of differentially expressed proteins in plasma - Identification and quantification of differentially expressed proteins in plasma
Swarup V, Srivastava AK, Rajeswari MR. Neurosci Res. 2012 Jun;73(2):161-7.
Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman - Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman
Wang Y, Song Z, Wagner JD, Pachuk C, Subramanian RR. J Lipid Res. 2011 Jun;52(6):1265-71.