Anti-Apoptosis inhibitor 5 antibody
| Name | Anti-Apoptosis inhibitor 5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab65836 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide from the C-terminus of human Apoptosis inhibitor 5, identical to the related rat and mouse sequence |
| Description | Rabbit Polyclonal |
| Gene | API5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This image shows human epidermal carcinoma tissue stained with ab65836 at 0.5-1µg/ml.
Anti-Apoptosis inhibitor 5 antibody (ab65836) at 2 µg/ml + rat cardiac muscle tissue lysate
ICC/IF image of ab65836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.