Anti-Aquaporin 1 antibody [1/22]
| Name | Anti-Aquaporin 1 antibody [1/22] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9566 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 1/22 |
| Applications | WB ELISA IHC-P IHC-F ICC/IF FC IHC-F |
| Species Reactivities | Mouse, Rat, Human, Sheep, Bovine |
| Antigen | Synthetic peptide: GQVEEYDLDDDINSRVEMKPK , corresponding to amino acids 249-269 of Aquaporin 1 |
| Description | Mouse Monoclonal |
| Gene | AQP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical staining of aquaporin 1 in rat brain.
![Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8787_Aquaporin-1-Primary-antibodies-ab9566-4.jpg)
Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/8788_Aquaporin-1-Primary-antibodies-ab9566-5.jpg)
Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
Product References
A human in vitro model that mimics the renal proximal tubule. - A human in vitro model that mimics the renal proximal tubule.
Hoppensack A, Kazanecki CC, Colter D, Gosiewska A, Schanz J, Walles H, Schenke-Layland K. Tissue Eng Part C Methods. 2014 Jul;20(7):599-609. doi:
Reciprocity in the developmental regulation of aquaporins 1, 3 and 5 during - Reciprocity in the developmental regulation of aquaporins 1, 3 and 5 during
Nazemi S, Rahbek M, Parhamifar L, Moghimi SM, Babamoradi H, Mehrdana F, Klaerke DA, Knight CH. PLoS One. 2014 Sep 3;9(9):e106809.
Architecture of kangaroo rat inner medulla: segmentation of descending thin limb - Architecture of kangaroo rat inner medulla: segmentation of descending thin limb
Urity VB, Issaian T, Braun EJ, Dantzler WH, Pannabecker TL. Am J Physiol Regul Integr Comp Physiol. 2012 Mar 15;302(6):R720-6. doi:
The water permeability channels aquaporins 1-4 are differentially expressed in - The water permeability channels aquaporins 1-4 are differentially expressed in
Thoroddsen A, Dahm-Kahler P, Lind AK, Weijdegard B, Lindenthal B, Muller J, Brannstrom M. J Clin Endocrinol Metab. 2011 Apr;96(4):1021-8.
The water permeability channels aquaporins 1-4 are differentially expressed in - The water permeability channels aquaporins 1-4 are differentially expressed in
Thoroddsen A, Dahm-Kahler P, Lind AK, Weijdegard B, Lindenthal B, Muller J, Brannstrom M. J Clin Endocrinol Metab. 2011 Apr;96(4):1021-8.
Aquaporin-1 in blood vessels of rat circumventricular organs. - Aquaporin-1 in blood vessels of rat circumventricular organs.
Wilson AJ, Carati CJ, Gannon BJ, Haberberger R, Chataway TK. Cell Tissue Res. 2010 Apr;340(1):159-68.
Agmatine attenuates brain edema through reducing the expression of aquaporin-1 - Agmatine attenuates brain edema through reducing the expression of aquaporin-1
Kim JH, Lee YW, Park KA, Lee WT, Lee JE. J Cereb Blood Flow Metab. 2010 May;30(5):943-9.
Atypical cystic meningioma overexpressing AQP1 in early infancy: case report with - Atypical cystic meningioma overexpressing AQP1 in early infancy: case report with
Marton E, Feletti A, Basaldella L, Dei Tos AP, Bendini M, Longatti P. Acta Paediatr. 2008 Aug;97(8):1145-9.
Expression and function of the Delta-1/Notch-2/Hes-1 pathway during experimental - Expression and function of the Delta-1/Notch-2/Hes-1 pathway during experimental
Kobayashi T, Terada Y, Kuwana H, Tanaka H, Okado T, Kuwahara M, Tohda S, Sakano S, Sasaki S. Kidney Int. 2008 Jun;73(11):1240-50.
V2 vasopressin receptor deficiency causes changes in expression and function of - V2 vasopressin receptor deficiency causes changes in expression and function of
Schliebe N, Strotmann R, Busse K, Mitschke D, Biebermann H, Schomburg L, Kohrle J, Bar J, Rompler H, Wess J, Schoneberg T, Sangkuhl K. Am J Physiol Renal Physiol. 2008 Oct;295(4):F1177-90. doi: