Anti-ARFBP1 antibody
| Name | Anti-ARFBP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70161 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Human, Chimpanzee, Ape, Orangutan |
| Antigen | A region between residues 2250 and 2300 of human ARFBP1 (NP_113584 |
| Description | Rabbit Polyclonal |
| Gene | HUWE1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling ARFBP1 with ab70161 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-ARFBP1 antibody (ab70161) at 1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 5 µg
ICC/IF image of ab70161 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab70161 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70161, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Autophagic degradation of the inhibitory p53 isoform Delta133p53alpha as a - Autophagic degradation of the inhibitory p53 isoform Delta133p53alpha as a
Horikawa I, Fujita K, Jenkins LM, Hiyoshi Y, Mondal AM, Vojtesek B, Lane DP, Appella E, Harris CC. Nat Commun. 2014 Aug 21;5:4706.
Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric - Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric
Izumi H, Kaneko Y. Cancer Res. 2014 Oct 1;74(19):5620-30.
Deciphering the transcriptional complex critical for RhoA gene expression and - Deciphering the transcriptional complex critical for RhoA gene expression and
Chan CH, Lee SW, Li CF, Wang J, Yang WL, Wu CY, Wu J, Nakayama KI, Kang HY, Huang HY, Hung MC, Pandolfi PP, Lin HK. Nat Cell Biol. 2010 May;12(5):457-67.