All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml (with 3% milk block)Lane 1 : Forebrain (Mouse) Tissue LysateLane 2 : Rat Cortex Tissue LysateLane 3 : Hippocampus (Mouse) Tissue Lysate Lane 4 : Rat Hippocampus Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/mlLane 1 : Hippocampus (Mouse) Tissue Lysate blocked with 5% BSALane 2 : Rat Hippocampus Tissue Lysate blocked with 5% BSALane 3 : Hippocampus (Mouse) Tissue Lysate blocked with 5% milkLane 4 : Rat Hippocampus Tissue Lysate blocked with 5% milkLane 5 : Hippocampus (Mouse) Tissue Lysate blocked with 3% milkLane 6 : Rat Hippocampus Tissue Lysate blocked with 3% milkLane 7 : Hippocampus (Mouse) Tissue Lysate blocked with 1% milkLane 8 : Rat Hippocampus Tissue Lysate blocked with 1% milkLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.