Anti-Arg2 antibody [EPR9473]

• Supplier: Abcam
• Catalog: ab137069
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9473
• Applications: ICC/IF ICC/IF FC WB
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues at the C terminus of Human Arg2
• Description: Rabbit Monoclonal
• Gene: ARG2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HEK293 cells stained with ab137069 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137069, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Arg2 antibody [EPR9473] (ab137069) at 1/1000 dilutionLane 1 : Caco 2 cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Immunofluorescent analysis of Caco 2 cells labelling Arg2 with ab137069 at 1/250 dilution.