Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al (2006).
All lanes : Anti-Argonaute 3 / eIF2C 3 antibody (ab3593) at 2 µg/mlLane 1 : Dorsal Root Ganglia (Rat) Tissue LysateLane 2 : Dorsal Root Ganglia (Mouse) Tissue LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ab3593 staining Argonaute 3 / eIF2C 3 in murine testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then blocked with 10% serum for 30 minutes at 25°C and then incubated with ab3593 at a 1/50 dilution for 1 hour 30 minutes at 37°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.See Abreview