Anti-ARMET antibody
| Name | Anti-ARMET antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab67271 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A 12 amino acid synthetic peptide from near the carboxy terminus of human ARMET |
| Description | Rabbit Polyclonal |
| Gene | MANF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-ARMET antibody (ab67271) at 1 µg/mlLane 2 : Anti-ARMET antibody (ab67271) at 2 µg/mlLane 1 : Mouse brain tissue lysateLane 2 : Mouse brain tissue lysateLysates/proteins at 15 µg per lane.SecondaryAnti rabbit secondary antibody
ab67271 at 2.5 μg/ml staining of ARMET in human brain tissue using IHC-P.
ICC/IF image of ab67271 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67271, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of MANF in Human Brain cells using ab67271 at 20 ug/ml.
Product References
Spatiotemporal expression of MANF in the developing rat brain. - Spatiotemporal expression of MANF in the developing rat brain.
Wang H, Ke Z, Alimov A, Xu M, Frank JA, Fang S, Luo J. PLoS One. 2014 Feb 28;9(2):e90433.
Armet/Manf and Creld2 are components of a specialized ER stress response provoked - Armet/Manf and Creld2 are components of a specialized ER stress response provoked
Hartley CL, Edwards S, Mullan L, Bell PA, Fresquet M, Boot-Handford RP, Briggs MD. Hum Mol Genet. 2013 Dec 20;22(25):5262-75.
Plasmid-based genetic modification of human bone marrow-derived stromal cells: - Plasmid-based genetic modification of human bone marrow-derived stromal cells:
Ronsyn MW, Daans J, Spaepen G, Chatterjee S, Vermeulen K, D'Haese P, Van Tendeloo VF, Van Marck E, Ysebaert D, Berneman ZN, Jorens PG, Ponsaerts P. BMC Biotechnol. 2007 Dec 14;7:90.