IHC image of ARMET staining in Human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab130736, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Anti-ARMET antibody [1G12] (ab130736) at 1/2000 dilution + Purified recombinant MANF expressed using CHOEBNALT85 cell line at 0.5 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/9676_ARMET-Primary-antibodies-ab130736-1.bmp)
Anti-ARMET antibody [1G12] (ab130736) at 1/2000 dilution + Purified recombinant MANF expressed using CHOEBNALT85 cell line at 0.5 µg