All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/5000 dilutionLane 1 : 293 cell lysateLane 2 : Caco-2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Aryl hydrocarbon Receptor antibody [EPR7119(N)(2)] (ab190797) at 1/1000 dilutionLane 1 : PC3 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Aryl hydrocarbon Receptor with ab190797 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®555) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue). Negative controls also shown.