Lane 1 : Anti-ASAH1 antibody (ab74469) at 1 µg/mlLane 2 : Anti-ASAH1 antibody (ab74469) at 2 µg/mlLane 1 : Mouse heart tissue lysateLane 2 : Mouse heart tissue lysateLysates/proteins at 15 µg per lane.Observed band size : 52 kDa (why is the actual band size different from the predicted?)Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
ab74469 at 2.5µg/ml staining ASAH1 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunofluorescence of ASAH1 in Human Heart cells using ab74469 at 20 ug/ml.