ASAH1

Gene Symbol ASAH1
Entrez Gene 427
Alt Symbol AC, ACDase, ASAH, PHP, PHP32, SMAPME
Species Human
Gene Type protein-coding
Description N-acylsphingosine amidohydrolase (acid ceramidase) 1
Other Description acid CDase|acid ceramidase|acylsphingosine deacylase|putative 32 kDa heart protein
Swissprots E9PDS0 Q6W898 Q13510 Q96AS2
Accessions AAF91230 BAG12319 BAG12320 BAG12321 BAG12322 BAG12323 BAG12324 BAG12325 BAG12326 BAG12327 BAG12328 BAG12329 BAG12330 BAG12331 BAG12332 BAG12333 BAG12334 BAG12335 BAG12336 BAG12337 BAG12338 BAG12339 BAG12340 BAG12341 BAG12342 BAG12343 BAG12344 BAG12345 BAG12346 BAG12347 BAG12348 BAG12349 BAG12350 BAG12351 BAG12352 BAG12353 BAG12354 BAG12355 BAG12356 BAG12357 BAG12358 BAG12359 BAG12360 BAG12361 BAG12362 BAG12363 BAG12364 BAG12365 BAG12366 BAG12367 BAG12368 BAG12369 BAG12370 BAG12371 BAG12372 BAG12373 BAG12374 BAG12375 BAG12376 BAG12377 EAW63791 EAW63792 EAW63793 EAW63794 EAW63795 Q13510 AK025211 BAB15085 AK025732 AK098299 BAG53608 AK222780 BAD96500 AK289481 BAF82170 AY305384 AAQ75550 BC016481 AAH16481 BC016828 AAH16828 BC035453 BG714023 BU633536 CB216602 DA260852 DA306573 DB561177 DQ891071 ABM81997 DQ894254 ABM85180 H78830 U47674 AAC73009 U70063 AAC50907 XM_005273504 XP_005273561 NM_001127505 NP_001120977 NM_004315 NP_004306 NM_177924 NP_808592
Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Subcellular Location Lysosome.
Tissue Specificity Broadly expressed with highest expression in heart.
Top Pathways Lysosome, Sphingolipid signaling pathway, Sphingolipid metabolism

Acid Ceramidase (T-20) - sc-28486 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Acid Ceramidase (T-20): sc-28486. Western blot analysis of Acid Ceramidase expression in HeLa whole cell lysate.

Acid Ceramidase (H-41) - sc-292176 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Acid Ceramidase (H-41): sc-292176. Western blot analysis of Acid Ceramidase expression in HeLa whole cell lysate.

Acid Ceramidase (23) - sc-136275 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Acid Ceramidase (23): sc-136275. Western blot analysis of Acid Ceramidase expression in NHEK whole cell lysate.

Anti-ASAH1 antibody - ab74469 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-ASAH1 antibody (ab74469) at 1 µg/mlLane 2 : Anti-ASAH1 antibody (ab74469) at 2 µg/mlLane 1 : Mouse heart tissue lysateLane 2 : Mouse heart tissue lysateLysates/proteins at 15 µg per lane.Observed band size : 52 kDa (why is the actual band size different from the predicted?)Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-ASAH1 antibody - ab54822 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ASAH1 antibody (ab54822) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.

Anti-ASAH1 antibody - ab154401 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ASAH1 antibody (ab154401) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

Anti-ASAH1 antibody [1A7] - ab124262 from Abcam

Prices $464.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
ab124262, at 5 µg/ml, staining ASAH1 in formalin fixed, paraffin embedded Human heart tissue by Imunohistochemistry.

Anti-ASAH1 antibody [EPR12108] - ab174828 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12108
All lanes : Anti-ASAH1 antibody [EPR12108] (ab174828) at 1/1000 dilutionLane 1 : Human fetal lung lysateLane 2 : Human skin lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-ASAH1 / Acid Ceramidase Antibody (clone 1A7) IHC-plus™ - LS-B6009 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
Anti-ASAH1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6009 concentration 5 ug/ml.

Anti-ASAH1 / Acid Ceramidase Antibody (N-Terminus) IHC-plus™ - LS-B11969 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-ASAH1 / Acid Ceramidase Antibody - LS-C309518 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ASAH1 / Acid Ceramidase Antibody (aa287-336) - LS-C107769 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ASAH1 antibody LS-C107769 Western blot of THP-1 cell lysate.

Anti-ASAH1 / Acid Ceramidase Antibody - LS-C334776 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using ASAH1 antibody.

Anti-ASAH1 / Acid Ceramidase Antibody (clone 2C9) - LS-C104888 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C9
Immunoperoxidase of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human heart (antibody concentration 3 ug/ml).

Anti-ASAH1 / Acid Ceramidase Antibody - LS-C335402 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of SH-SY5Y cell line, using ASAH1 antibody.

Anti-ASAH1 / Acid Ceramidase Antibody (C-Terminus) IHC-plus™ - LS-B3136 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ASAH1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3136 concentration 5 ug/ml.

ASAH1 Antibody - NBP1-76933 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: ASAH1 Antibody [NBP1-76933] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.

ASAH1 Antibody - NBP1-89296 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ASAH1 Antibody [NBP1-89296] - Analysis in human cell line SK-MEL-30.

ASAH1 Antibody (1A7) - H00000427-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
Western Blot: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of ASAH1 expression in transfected 293T cell line by ASAH1 monoclonal antibody (M02), clone 1A7.Lane 1: ASAH1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

ASAH1 Antibody - NBP2-15479 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ASAH1 Antibody (2C9) - H00000427-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C9
Immunohistochemistry-Paraffin: ASAH1 Antibody (2C9) [H00000427-M01] - Analysis of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

ASAH1 Antibody - H00000427-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ASAH1 Polyclonal Antibody - bs-12976R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ASAH1 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-12976R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ASAH1 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-12976R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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