Anti-ASH2L antibody [2046D2a]
| Name | Anti-ASH2L antibody [2046D2a] |
|---|---|
| Supplier | Abcam |
| Catalog | ab50699 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2046D2a |
| Applications | FC WB ICC/IF DB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment |
| Description | Mouse Monoclonal |
| Gene | ASH2L |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + HeLa whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10172_ash2a.jpg)
Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + HeLa whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique
HeLa cells labelled with ab50699 at 1/5 dilution.Secondary antibody: Alexa 488 Goat Anti-Mouse IgG at 1/200.
![Anti-ASH2L antibody [2046D2a] (ab50699) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10174_ash4.jpg)
Anti-ASH2L antibody [2046D2a] (ab50699) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique
![Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + F2408 whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10175_ash5.jpg)
Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + F2408 whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique
![Overlay histogram showing HeLa cells stained with ab50699 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50699, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10176_ASH2L-Primary-antibodies-ab50699-5.jpg)
Overlay histogram showing HeLa cells stained with ab50699 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50699, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
In situ histone landscape of nephrogenesis. - In situ histone landscape of nephrogenesis.
McLaughlin N, Wang F, Saifudeen Z, El-Dahr SS. Epigenetics. 2014 Feb;9(2):222-35.
The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and - The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and
Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey TN, Meyer B, Varagnolo L, Muller AM, Karas M, Dingermann T, Marschalek R. Leukemia. 2011 Jan;25(1):135-44.
The 19S proteasome positively regulates histone methylation at cytokine inducible - The 19S proteasome positively regulates histone methylation at cytokine inducible
Koues OI, Dudley RK, Mehta NT, Greer SF. Biochim Biophys Acta. 2009 Nov-Dec;1789(11-12):691-701. doi: