ASH2L

Gene Symbol	ASH2L
Entrez Gene	9070
Alt Symbol	ASH2, ASH2L1, ASH2L2, Bre2
Species	Human
Gene Type	protein-coding
Description	ash2 (absent, small, or homeotic)-like (Drosophila)
Other Description	ASH2-like protein\|set1/Ash2 histone methyltransferase complex subunit ASH2
Swissprots	Q96B62 O60659 D3DSW9 A8K7C3 Q9UBL3 O60660
Accessions	BAA74520 EAW63336 EAW63337 EAW63338 EAW63339 EAW63340 Q9UBL3 AB020982 BAA35127 AF056717 AAC13563 AF056718 AAC13564 AK291750 BAF84439 AK291938 BAF84627 AK293699 BAG57134 AK298483 BAG60693 AK311336 AW962440 BC015936 AAH15936 CA417078 DA156139 DC326351 DC353644 DC396707 XM_005273682 XP_005273739 XM_005273683 XP_005273740 XM_006716412 XP_006716475 XM_006716413 XP_006716476 NM_001105214 NP_001098684 NM_001261832 NP_001248761 NM_001282272 NP_001269201 NM_004674 NP_004665
Function	Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. {ECO:0000269\|PubMed:12670868, ECO:0000269\|PubMed:19556245}.
Subcellular Location	Nucleus {ECO:0000305\|PubMed:17355966}.
Tissue Specificity	Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. {ECO:0000269\|PubMed:10393421}.

ASH2L (D93F6) XP ® Rabbit mAb - 5019 from Cell Signaling Technology

Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (20 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D93F6

Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP ® Rabbit mAb.

ASH2L (F-19) - sc-27283 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ASH2L (F-19): sc-27283. Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing nuclear staining of glandular cells.

ASH2L (H-300) - sc-367190 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

ASH2L (H-300): sc-367190. Western blot analysis of ASH2L expression in non-transfected: sc-117750 (A) and mouse ASH2L transfected: sc-118586 (B) whole cell lysates.

ASH2L (2046D2a) - sc-81184 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

ASH2 (2046D2a): sc-81184 Western Blot analysis of ASH2 expression in F2408 whole cell lysate.

Anti-ASH2L antibody - ab119414 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L antibody (ab119414) at 1/500 dilution + HL60 cell extract

Anti-ASH2L antibody - ab84732 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L antibody (ab84732) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-ASH2L antibody - ab28426 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L antibody (ab28426) at 0.25 µg/ml + Jurkat cell lysateSecondaryHRP-conjugated anti rabbit IgG diluted 1/50,000 - 1/100,000

Anti-ASH2L antibody - ab84407 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma, using 1/250 ab84407 followed by DAB staining.

Anti-ASH2L antibody - ab89444 from Abcam

Host	Mouse
Clonality	Monoclonal

Anti-ASH2L antibody (ab89444) at 1/500 dilution + Jurkat cell lysate at 50 µg

Anti-ASH2L antibody - ab192906 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L antibody (ab192906) at 1 µg/ml + HepG2 whole cell lysate at 10 µg

Anti-ASH2L antibody [2046D2a] - ab50699 from Abcam

Prices	$380.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2046D2a

Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + HeLa whole cell lysate at 25 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique

Anti-ASH2L antibody [EPR13106] - ab181117 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13106

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).

Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker - ab176334 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13107(B)

Immunofluorescent analysis of HeLa labeling ASH2Lwith ab176334 at 1/100 dilution.

Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (Alexa Fluor® 488) - ab184815 from Abcam

Prices	$438.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13107(B)

Overlay histogram showing HeLa cells stained with ab184815 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184815, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-ASH2L / ASH2 Antibody - LS-C48695 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody (aa60-120) - LS-C69313 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody (C-Terminus) - LS-C69316 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody (aa228-257) - LS-C69315 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody - LS-C69317 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal

Anti-ASH2L / ASH2 Antibody (aa54-103) - LS-C29858 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody (aa54-103) - LS-C31349 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody - LS-C126895 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal

Anti-ASH2L / ASH2 Antibody - LS-C341782 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ASH2L / ASH2 Antibody (aa36-85) - LS-C112162 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ASH2L antibody LS-C112162 Western blot of HeLa lysate.

Anti-ASH2L / ASH2 Antibody (C-Terminus) - LS-C287375 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.

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