
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).

Immunofluorescent analysis of acetone fixed HeLa cells labeling ASH2L with ab181117 at 1/100 (green). Staining with Dapi is shown in blue.

Immunoprecipitation analysis of 293T cells labeling ASH2L using ab181117 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.
![All lanes : Anti-ASH2L antibody [EPR13106] (ab181117) at 1/10000 dilutionLane 1 : 293T cell line lysateLane 2 : K562 cell line lysateLane 3 : C6 cell line lysateLane 4 : PC12 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10180_ab181117-213392-ab181117WB.JPG)
All lanes : Anti-ASH2L antibody [EPR13106] (ab181117) at 1/10000 dilutionLane 1 : 293T cell line lysateLane 2 : K562 cell line lysateLane 3 : C6 cell line lysateLane 4 : PC12 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution