Anti-Asporin antibody
| Name | Anti-Asporin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58741 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Pig, Zebrafish |
| Antigen | A region within synthetic peptide: ISTVELEDFK RYKELQRLGL GNNKITDIEN GSLANIPRVR EIHLENNKLK, corresponding to internal sequence amino acids 253-302 of Human Asporin Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | ASPN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Asporin antibody (ab58741) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with skim milk in PBS buffer at 5 %SecondaryHRP conjugated anti-Rabbit IgG at 1: 50,000 - 100,000
ICC/IF image of ab58741 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58741, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Asporin with ab58741 at a concentration of 4-8µg/ml. Skeletal muscle cells (indicated by arrows) were positively stained. Magnification: 400X.