Anti-ASPP1 antibody
| Name | Anti-ASPP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab71163 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | A synthetic peptide corresponding to a region between residue 50 and 100 of human ASPP1 (NP_056131 |
| Description | Rabbit Polyclonal |
| Gene | PPP1R13B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ASPP1 antibody (ab71163) at 0.1 µg/mlLane 1 : Whole cell lysate from 293T cells at 50 µgLane 2 : Whole cell lysate from 293T cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 5 µgLane 4 : Whole cell lysate from Hela cells at 50 µgdeveloped using the ECL technique
Immunoprecipitation/ Western Blot of ASPP1. Lane 1: ab71163 at 3µg/mg whole cell lysate. Lane 2: Control IgG. All lanes: Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 10 seconds.
ICC/IF image of ab71163 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71163, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3. - E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3.
Chen D, Chen Y, Forrest D, Bremner R. Cell Death Differ. 2013 Jul;20(7):931-40.
An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular - An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular
Vigneron AM, Vousden KH. EMBO J. 2012 Jan 18;31(2):471-80.