Anti-ASPP1 antibody
| Name | Anti-ASPP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51831 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide corresponding to an internal sequence of ASPP1 (Human) |
| Description | Rabbit Polyclonal |
| Gene | PPP1R13B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ASPP1 antibody (ab51831) at 1/1000 dilutionLane 1 : Non-transfected controlLane 2 : MCF7 cells over expressing ASPP1Lane 3 : MCF7 cells overexpressing ASPP2
ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.