All lanes : Anti-ASTL antibody (ab59889) at 1/1000 dilutionLane 1 : Tissue lysate prepared from rat ovary (5 mg/ml) at 35 µlLane 2 : Tissue lysate prepared from rat ovary (5 mg/ml) at 20 µlLane 3 : Tissue lysate prepared from rat ovary (5 mg/ml) at 10 µlLane 4 : Tissue lysate prepared from rat ovary (5 mg/ml) at 5 µlLane 5 : Tissue lysate prepared from rat ovary (5 mg/ml) at 2 µlLane 6 : Tissue lysate prepared from mouse ovary(5 mg/ml) at 35 µlLane 7 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 20 µlLane 8 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 10 µlLane 9 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 5 µlLane 10 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 2 µl