ASTL

Gene Symbol ASTL
Entrez Gene 431705
Alt Symbol SAS1B
Species Human
Gene Type protein-coding
Description astacin-like metallo-endopeptidase (M12 family)
Other Description astacin-like metalloendopeptidase|astacin-like metalloendopeptidase (M12 family)|oocyte astacin|ovastacin|sperm acrosomal SLLP1 binding
Swissprots Q3KNT0 Q6HA08
Accessions EAW71389 Q6HA08 AJ537600 CAD61265 BC107126 AAI07127 BC107127 AAI07128 XM_011511205 XP_011509507 XM_011511206 XP_011509508 XM_011511207 XP_011509509 XM_011511208 XP_011509510 NM_001002036 NP_001002036
Function Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle {ECO:0000250}. Note=Secretory granules. Localizes to the peripheral cortical granules of ovulated eggs. Probably exocytosed from cortical granules during post- fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles shortly after birth. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS). Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS) (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues. {ECO:0000269|PubMed:15087446}.

ASTL (E-16) - sc-107340 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASTL (E-16): sc-107340. Western blot analysis of ASTL expression in non-transfected: sc-117752 (A) and mouse ASTL transfected: sc-126457 (B) 293T whole cell lysates.

ASTL (M-12) - sc-107343 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASTL (M-12): sc-107343. Western blot analysis of ASTL expression in non-transfected: sc-117752 (A) and mouse ASTL transfected: sc-126457 (B) 293T whole cell lysates.

ASTL (G-18) - sc-107341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASTL (G-18): sc-107341. Western blot analysis of ASTL expression in rat ovary (A) and mouse ovary (B) tissue extracts and NIH/3T3 whole cell lysate (C).

ASTL (N-12) - sc-107344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ASTL (N-12): sc-107344. Western blot analysis of ASTL expression in non-transfected: sc-117752 (A) and mouse ASTL transfected: sc-126457 (B) 293T whole cell lysates.

ASTL (K-15) - sc-107342 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-ASTL antibody - ab59889 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ASTL antibody (ab59889) at 1/1000 dilutionLane 1 : Tissue lysate prepared from rat ovary (5 mg/ml) at 35 µlLane 2 : Tissue lysate prepared from rat ovary (5 mg/ml) at 20 µlLane 3 : Tissue lysate prepared from rat ovary (5 mg/ml) at 10 µlLane 4 : Tissue lysate prepared from rat ovary (5 mg/ml) at 5 µlLane 5 : Tissue lysate prepared from rat ovary (5 mg/ml) at 2 µlLane 6 : Tissue lysate prepared from mouse ovary(5 mg/ml) at 35 µlLane 7 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 20 µlLane 8 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 10 µlLane 9 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 5 µlLane 10 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 2 µl

Anti-ASTL antibody - ab169975 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ASTL antibody (ab169975) at 1/500 dilution + 293T cell lysate

Anti-ASTL antibody - Catalytic domain - ab59895 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ASTL antibody - Catalytic domain (ab59895) at 1/1000 dilutionLane 1 : Tissue lysate prepared from rat ovary (5 mg/ml) at 35 µlLane 2 : Tissue lysate prepared from rat ovary (5 mg/ml) at 20 µlLane 3 : Tissue lysate prepared from rat ovary (5 mg/ml) at 10 µlLane 4 : Tissue lysate prepared from rat ovary (5 mg/ml) at 5 µlLane 5 : Tissue lysate prepared from rat ovary (5 mg/ml) at 2 µlLane 6 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 35 µlLane 7 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 20 µlLane 8 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 10 µlLane 9 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 5 µlLane 10 : Tissue lysate prepared from mouse ovary (5 mg/ml) at 2 µl

astacin-like metalloendopeptidase Antibody - NBP1-90683 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: astacin-like metalloendopeptidase Antibody [NBP1-90683] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG

Anti-ASTL antibody produced in rabbit - HPA015620 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ASTL: <B>Cat. No. HPA015620</B>: Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.

Human Ovastacin Antibody - AF8549 from R&D Systems

Prices $359.00
Sizes 100 µl
Host Rabbit
Detection of Human Ovastacin by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008 ). A specific band was detected for Ovastacin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

ASTL Antibody - abx037138 from Abbexa

Host Rabbit
Clonality Polyclonal

ASTL antibody - 21418-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21418-1-AP( ASTL Antibody) at dilution of 1:300

ASTL antibody - 21418-1-AP from Proteintech Group

Prices $279.00
Sizes con: 30 μg/150μl
Host Rabbit
Clonality Polyclonal
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21418-1-AP( ASTL Antibody) at dilution of 1:300

ASTL Antibody - 39946 from Signalway Antibody

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ASTL Antibody - E306719 from Enogene

Prices $275.00
Sizes 100ug/200ul

ASTL , Polyclonal Antibody - MBS859319 from MyBioSource

Prices $330.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ASTL, Polyclonal Antibody - MBS417725 from MyBioSource

Prices $230.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Rabbit Anti-Human ASTL Polyclonal antibody - DPABH-08368 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Monoclonal Antibody to Astacin Like Metallo Endopeptidase (ASTL) - MAJ593Hu21 from Uscn Life Science Inc.

Host Mouse
Clonality Monoclonal

Polyclonal Antibody to Astacin Like Metallo Endopeptidase (ASTL) - PAJ593Hu01 from Uscn Life Science Inc.

Host Rabbit
Clonality Polyclonal

Polyclonal Antibody to Astacin Like Metallo Endopeptidase (ASTL) - PAJ593Hu01 from Cloud-clone

Host Rabbit
Clonality Polyclonal

Anti-ASTL Antibody (aa228-407) - LS-C348907 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot of ASTL antibody

astacin-like metalloendopeptidase Antibody [Unconjugated] - AF8549 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ovastacin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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