All lanes : Anti-Ataxin 1 (phospho S776) antibody (ab63376) at 1/500 dilutionLane 1 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours)Lane 2 : HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) with immunizing phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.
ab63376 staining Ataxin 1 (phospho S776) in human HEK cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.01% Triton X-100 and then blocked using 30% goat serum for 30 minutes at 37°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 37°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 594 (red) used at a 1/400 dilution.See Abreview