Anti-ATF-4 antibody

• Supplier: Abcam
• Catalog: ab23760
• Prices: $382.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Human, Mouse, Rat
• Antigen: Synthetic peptide corresponding to Human ATF-4 aa 300 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH)
• Description: Rabbit Polyclonal
• Gene: ATF4
• Conjugate: Unconjugated

Product images

Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilutionPerformed under reducing conditions.

ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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