ATF4

Gene Symbol ATF4
Entrez Gene 468
Alt Symbol CREB-2, CREB2, TAXREB67, TXREB
Species Human
Gene Type protein-coding
Description activating transcription factor 4
Other Description DNA-binding protein TAXREB67|cAMP response element-binding protein 2|cAMP-dependent transcription factor ATF-4|cAMP-responsive element-binding protein 2|cyclic AMP-dependent transcription factor ATF-4|cyclic AMP-responsive element-binding protein 2|tax-responsive enhancer element B67|tax-responsive enhancer element-binding protein 67
Swissprots Q9UH31 P18848
Accessions EAW60341 EAW60342 P18848 AK057751 AK296028 BAG58796 BC008090 AAH08090 BC011994 AAH11994 BC016855 AAH16855 BC022088 AAH22088 BC024775 AAH24775 BC044895 AAH44895 BC073754 AAH73754 BC073990 AAH73990 CR450353 CAG29349 CR456384 CAG30270 CU012942 CAK54373 CU013230 CAK54672 D90209 BAA14234 DQ891758 ABM82684 DQ894942 ABM85868 M86842 AAA52071 NM_001675 NP_001666 NM_182810 NP_877962
Function Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. {ECO:0000
Subcellular Location Cytoplasm. Cell membrane. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Co- localizes with NEK6 in the centrosome. {ECO:0000250}.
Top Pathways MAPK signaling pathway, cGMP-PKG signaling pathway, Thyroid hormone synthesis, Adrenergic signaling in cardiomyocytes, Prostate cancer

ATF-4 (D4B8) Rabbit mAb - 11815 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4B8
Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or tunicamycin-treated (2 μg/ml, 8 hr; +), using ATF-4 (D4B8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

CREB-2 (B-3) - sc-390063 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CREB-2 (B-3): sc-390063. Western blot analysis of CREB-2 expression in K-562 (A) and Jurkat (B) whole cell lysates and K-562 (C) and Jurkat (D) nuclear extracts.

CREB-2 (H-290) - sc-22800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CREB-2 (H-290): sc-22800. Western blot analysis of CREB-2 expression in HeLa nuclear extract (A) and A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.

CREB-2 (C-19) - sc-7583 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CREB-2 (C-19): sc-7583. Western blot analysis of CREB-2 expression in HeLa (A) and A-431 (B) whole cell lysates and A-431 nuclear extract (C).

CREB-2 (C-20) - sc-200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CREB-2 (C-20): sc-200. Western blot analysis of CREB-2 expression in K-562 (A) and A-431 (B) nuclear extracts.

p-CREB-2 (Ser 245) - sc-101639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-CREB-2 (Ser 245): sc-101639. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue extract showing nuclear localization.

Anti-ATF-4 (phospho S219) antibody - ab192580 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ATF-4 (phospho S219) antibody (ab192580) at 1/500 dilutionLane 1 : COLO cell lysateLane 2 : COLO cell lysate with antigen specific peptide

Anti-ATF-4 (phospho S245) antibody - ab28830 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab28830 staining ATF-4 (phospho S245) in Human MRC5 cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview

Anti-ATF-4 antibody - ab23760 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilutionPerformed under reducing conditions.

Anti-ATF-4 antibody - ab31390 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab31390 at a 1:50-1:100 dilution staining ATF-4 in human breast carcinoma tissue, using Immunohistochemistry, Paraffin Embedded Tissue.Left image : Un-treated.Right image : Antibody pre-incubated with synthesized peptide.

Anti-ATF-4 antibody - ab1371 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-ATF-4 antibody (ab1371) at 0.3 µg/ml + K562 cell lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-ATF-4 antibody [mAbcam50546] - ab50546 from Abcam

Prices $398.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam50546
All lanes : Anti-ATF-4 antibody [mAbcam50546] (ab50546) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ATF-4 antibody [S360A-24] (Biotin) - ab186297 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S360A-24

Anti-ATF-4 antibody [S360A-24] (FITC) - ab186256 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S360A-24

Anti-ATF-4 antibody [S360A-24] (HRP) - ab186268 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S360A-24

Anti-ATF-4 antibody [S360A24] - ab186284 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S360A24

Anti-ATF-4 antibody - ChIP Grade - ab85049 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ChIP analysis using ab85049 binding ATF-4 in human macrophages. Samples were incubated with undiluted primary antibody for 12 hours at 4°C. Protein binding was detected using real-time PCR.Negative Control: Normal rabbit IgG.See Abreview

Anti-ATF4 Antibody - LS-C155076 from LifeSpan Bioscience

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ATF4 Antibody (phospho-Ser245) - LS-C190436 from LifeSpan Bioscience

Prices $485.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ATF4 Antibody (clone 2B3) - LS-C139109 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B3
Detection limit for recombinant GST tagged ATF4 is 0.1 ng/ml as a capture antibody.

Anti-ATF4 Antibody (Ser245, AP) - LS-C206368 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATF4 Antibody (Ser245, APC) - LS-C206369 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATF4 Antibody (Ser245, Biotin) - LS-C206370 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATF4 Antibody (Ser245, FITC) - LS-C206371 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ATF4 Antibody (Ser245) - LS-C100013 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of ATF4 Antibody (S245) antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified antibody.
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