Anti-ATF6 antibody

• Supplier: Abcam
• Catalog: ab37149
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ELISA ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to Human ATF6 (N terminal)
• Blocking Peptide: Active human ATF6 peptide
• Description: Rabbit Polyclonal
• Gene: ATF6
• Conjugate: Unconjugated

Product images

ab37149 staining human Hella cells by ICC/IF. Cells were PFA fixed and permeabilized for 10 minutes in 0.1% Triton X-100 prior to blocking in 3% BSA for 15 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. The secondary antibody used was Alexa Fluor®568 goat anti-rabbit (ab175471) diluted at 1/1000.See Abreview

Lane 1 : Anti-ATF6 antibody (ab37149) at 0.5 µg/mlLane 2 : Anti-ATF6 antibody (ab37149) at 1 µg/mlLane 1 : MDA MB 361 cell lysateLane 2 : MDA MB 361 cell lysateLysates/proteins at 15 µg per lane.Secondarygoat anti-rabbit HRP at 1/10000 dilution

ab37149 staining human normal pancreas. Staining is localised to the cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Product References

beta-cell dysfunction due to increased ER stress in a stem cell model of Wolfram - beta-cell dysfunction due to increased ER stress in a stem cell model of Wolfram

Shang L, Hua H, Foo K, Martinez H, Watanabe K, Zimmer M, Kahler DJ, Freeby M, Chung W, LeDuc C, Goland R, Leibel RL, Egli D. Diabetes. 2014 Mar;63(3):923-33.

Impairment of visual function and retinal ER stress activation in Wfs1-deficient - Impairment of visual function and retinal ER stress activation in Wfs1-deficient

Bonnet Wersinger D, Benkafadar N, Jagodzinska J, Hamel C, Tanizawa Y, Lenaers G, Delettre C. PLoS One. 2014 May 13;9(5):e97222.

Oral treatment targeting the unfolded protein response prevents neurodegeneration - Oral treatment targeting the unfolded protein response prevents neurodegeneration

Moreno JA, Halliday M, Molloy C, Radford H, Verity N, Axten JM, Ortori CA, Willis AE, Fischer PM, Barrett DA, Mallucci GR. Sci Transl Med. 2013 Oct 9;5(206):206ra138.

Allelic heterogeneity contributes to variability in ocular dysgenesis, myopathy - Allelic heterogeneity contributes to variability in ocular dysgenesis, myopathy

Kuo DS, Labelle-Dumais C, Mao M, Jeanne M, Kauffman WB, Allen J, Favor J, Gould DB. Hum Mol Genet. 2014 Apr 1;23(7):1709-22.

Caspase-7 ablation modulates UPR, reprograms TRAF2-JNK apoptosis and protects - Caspase-7 ablation modulates UPR, reprograms TRAF2-JNK apoptosis and protects

Choudhury S, Bhootada Y, Gorbatyuk O, Gorbatyuk M. Cell Death Dis. 2013 Mar 7;4:e528.

12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response. - 12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response.

Cole BK, Kuhn NS, Green-Mitchell SM, Leone KA, Raab RM, Nadler JL, Chakrabarti SK. Am J Physiol Endocrinol Metab. 2012 Mar 15;302(6):E654-65. doi:

Oxidative processing of latent Fas in the endoplasmic reticulum controls the - Oxidative processing of latent Fas in the endoplasmic reticulum controls the

Anathy V, Roberson E, Cunniff B, Nolin JD, Hoffman S, Spiess P, Guala AS, Lahue KG, Goldman D, Flemer S, van der Vliet A, Heintz NH, Budd RC, Tew KD, Janssen-Heininger YM. Mol Cell Biol. 2012 Sep;32(17):3464-78.

Rotavirus infection activates the UPR but modulates its activity. - Rotavirus infection activates the UPR but modulates its activity.

Zambrano JL, Ettayebi K, Maaty WS, Faunce NR, Bothner B, Hardy ME. Virol J. 2011 Jul 20;8:359.

Nelfinavir induces liposarcoma apoptosis through inhibition of regulated - Nelfinavir induces liposarcoma apoptosis through inhibition of regulated

Guan M, Fousek K, Jiang C, Guo S, Synold T, Xi B, Shih CC, Chow WA. Clin Cancer Res. 2011 Apr 1;17(7):1796-806.

Bortezomib induction of C/EBPbeta mediates Epstein-Barr virus lytic activation in - Bortezomib induction of C/EBPbeta mediates Epstein-Barr virus lytic activation in

Shirley CM, Chen J, Shamay M, Li H, Zahnow CA, Hayward SD, Ambinder RF. Blood. 2011 Jun 9;117(23):6297-303.