Anti-ATF6 antibody (ab135707) at 1/100 dilution + A2058 cell line lysates at 35 µg
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labelling ATF6 with ab135707 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of K562 cells labelling ATF6 with ab135707 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.