Anti-ATF6 antibody [1-7]

• Supplier: Abcam
• Catalog: ab122897
• Prices: $398.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 1-7
• Applications: WB IP
• Species Reactivities: Human
• Antigen: Recombinant fragment (His-tag) corresponding to Human ATF6 (N terminal)
• Description: Mouse Monoclonal
• Gene: ATF6
• Conjugate: Unconjugated

Product images

All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilutionLane 1 : 293T cell lysate: controlLane 2 : 293T cell lysate: treated with 2 µg/ml tunicamycin for 7 hoursLane 3 : 293T cell lysate: treated with 1mM DTT for 1 hour.Lane 4 : HeLa S3 Tet-off cell lysate: controlLane 5 : HeLa S3 Tet-off cell lysate: treated with 1mM DTT for 1 hour.

All lanes : Anti-ATF6 antibody [1-7] (ab122897) at 1/500 dilutionLane 1 : HeLa cell extract: controlLane 2 : HeLa cell extract: treated with 1mM DTT for 1 hourLane 3 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 3 hourLane 4 : HeLa cell extract: treated with 2 µg/ml tunicamycin for 7 hourLane 5 : 293 cell extract: controlLane 6 : 293 cell extract: treated with 1mM DTT for 1 hourLane 7 : 293 cell extract: treated with 2 µg/ml tunicamycin for 3 hourLane 8 : 293 cell extract: treated with 2 µg/ml tunicamycin for 7 hour

ATF6 was detected by Western blot (Input; lanes 1, 2, 7, and 8) using ab122897 (No. 1-7). After immunoprecipitation (IP) with non-related IgG (IP; lanes 3, 4, 9, and 10) or ab122897 (No. 1-7) (IP; lanes 5, 6, 11, and 12), samples were subjected to SDS-PAGE and analyzed by Western blot using ab122897 (No. 1-7) and anti-mouse IgG antibody (light chain specific).

Product References

Targeted protein destabilization reveals an estrogen-mediated ER stress response. - Targeted protein destabilization reveals an estrogen-mediated ER stress response.

Raina K, Noblin DJ, Serebrenik YV, Adams A, Zhao C, Crews CM. Nat Chem Biol. 2014 Nov;10(11):957-62.

Selective activation of the transcription factor ATF6 mediates endoplasmic - Selective activation of the transcription factor ATF6 mediates endoplasmic

Maiuolo J, Bulotta S, Verderio C, Benfante R, Borgese N. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7832-7. doi:

Divest yourself of a preconceived idea: transcription factor ATF6 is not a - Divest yourself of a preconceived idea: transcription factor ATF6 is not a

Mori K. Mol Biol Cell. 2010 May 1;21(9):1435-8.