Anti-ATF6 antibody [70B1413] - ChIP Grade
| Name | Anti-ATF6 antibody [70B1413] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab11909 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 70B1413 |
| Applications | ICC/IF ICC/IF IHC-P IHC-F IP ChIP FC WB |
| Species Reactivities | Mouse, Rat, Rabbit, Hamster, Human |
| Antigen | Recombinant fragment corresponding to Human ATF6 aa 1-273 |
| Description | Mouse Monoclonal |
| Gene | ATF6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab11909 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11909, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab11909 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11909, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10677_ATF6-Primary-antibodies-ab11909-2.jpg)
Overlay histogram showing HeLa cells stained with ab11909 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11909, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
The unfolded protein response and the phosphorylations of activating - The unfolded protein response and the phosphorylations of activating
Rodriguez M, Domingo E, Alonso S, Frade JG, Eiros J, Crespo MS, Fernandez N. J Biol Chem. 2014 Aug 15;289(33):22942-57.
Secretory defect and cytotoxicity: the potential disease mechanisms for the - Secretory defect and cytotoxicity: the potential disease mechanisms for the
Li S, Yang Z, Hu J, Gordon WC, Bazan NG, Haas AL, Bok D, Jin M. J Biol Chem. 2013 Apr 19;288(16):11395-406.
Integration of the unfolded protein and oxidative stress responses through - Integration of the unfolded protein and oxidative stress responses through
Glover-Cutter KM, Lin S, Blackwell TK. PLoS Genet. 2013;9(9):e1003701.
Tick-borne encephalitis virus triggers inositol-requiring enzyme 1 (IRE1) and - Tick-borne encephalitis virus triggers inositol-requiring enzyme 1 (IRE1) and
Yu C, Achazi K, Niedrig M. Virus Res. 2013 Dec 26;178(2):471-7.
The endoplasmic reticulum-resident chaperone heat shock protein 47 protects the - The endoplasmic reticulum-resident chaperone heat shock protein 47 protects the
Miyata S, Mizuno T, Koyama Y, Katayama T, Tohyama M. PLoS One. 2013 Jul 29;8(7):e69732.
Pharmacological chaperoning of nicotinic acetylcholine receptors reduces the - Pharmacological chaperoning of nicotinic acetylcholine receptors reduces the
Srinivasan R, Richards CI, Xiao C, Rhee D, Pantoja R, Dougherty DA, Miwa JM, Lester HA. Mol Pharmacol. 2012 Jun;81(6):759-69.
Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage - Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage
Galligan JJ, Smathers RL, Shearn CT, Fritz KS, Backos DS, Jiang H, Franklin CC, Orlicky DJ, Maclean KN, Petersen DR. J Toxicol. 2012;2012:207594.
Antiviral activity of a small molecule deubiquitinase inhibitor occurs via - Antiviral activity of a small molecule deubiquitinase inhibitor occurs via
Perry JW, Ahmed M, Chang KO, Donato NJ, Showalter HD, Wobus CE. PLoS Pathog. 2012;8(7):e1002783.
Indomethacin induces apoptosis in 786-O renal cell carcinoma cells by activating - Indomethacin induces apoptosis in 786-O renal cell carcinoma cells by activating
Ou YC, Yang CR, Cheng CL, Raung SL, Hung YY, Chen CJ. Eur J Pharmacol. 2007 Jun 1;563(1-3):49-60. Epub 2007 Feb 8.