![ATG16L1 was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to ATG16L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47946.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 57kDa; ATG16L1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/10707_ab47946-197836-IPV023ab4794620minMod.jpg)
ATG16L1 was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to ATG16L1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47946.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 57kDa; ATG16L1
All lanes : Anti-ATG16L1 antibody (ab47946) at 1 µg/mlLane 1 : Lung (Human) Tissue Lysate Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) developed using the ECL techniquePerformed under reducing conditions.