Anti-ATG4C antibody - N-terminal (ab173796) at 1/100 dilution + Jurkat cell line lysate at 35 µg
Anti-ATG4C antibody - N-terminal (ab173796) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg
All lanes : Anti-ATG4C antibody - N-terminal (ab173796) at 1/60 dilutionLane 1 : Non-transfected 293 cell line lysatesLane 2 : 293 cell line lysates transiently transfected with the ATG4C geneLysates/proteins at 2 µg per lane.
Immunohistochemistry analysis of formalin-fixed and paraffin-embedded Human cancer tissue, labeling ATG4C with ab173796 at 1/50 dilution. Peroxidase-conjugaion to the secondary antibody, followed by AEC staining.