Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 at a 1/100 dilution (green). DAPI nuclear staining (blue).
All lanes : Anti-ATP5A antibody [EPR13030(B)] (ab176569) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Human fetal liver lysateLane 4 : Human fetal lung lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 at a 1/50 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 at a 1/10 dilution (red) or a rabbit IgG negative control (green).