ATP5A1

Gene Symbol	ATP5A1
Entrez Gene	498
Alt Symbol	ATP5A, ATP5AL2, ATPM, COXPD22, HEL-S-123m, MC5DN4, MOM2, OMR, ORM, hATP1
Species	Human
Gene Type	protein-coding
Description	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Other Description	ATP synthase alpha chain, mitochondrial\|ATP synthase subunit alpha, mitochondrial\|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle\|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2\|ATP sythase (F1-ATPase) alpha subunit\|epididymis secretory sperm binding protein Li 123m\|mitochondrial ATP synthetase, oligomycin-resistant
Swissprots	A8K092 Q96IR6 B4DY56 Q53XX6 K7ENP3 Q9BTV8 Q8IXV2 Q96HW2 P25705 Q96FB4
Accessions	BAA05672 CAA48804 EAX01471 P25705 AK092735 BAG52604 AK098765 AK129739 AK129804 AK129900 AK289457 BAF82146 AK294804 BAG57924 AK302272 BAG63618 AK310051 AU120644 AU143001 AW205533 AW207528 BC003119 AAH03119 BC007299 AAH07299 BC008028 AAH08028 BC011384 AAH11384 BC016046 AAH16046 BC019310 AAH19310 BC028335 BC035703 BC039135 AAH39135 BC042694 BC064562 AAH64562 BC067385 AAH67385 BT007209 AAP35873 BT008250 AAP36942 CD244623 CK002707 D14710 BAA03531 DQ890557 ABM92174 DQ894210 ABM85136 FJ224309 ACI46001 X59066 CAA41789 X65460 CAA46452 XM_011526018 XP_011524320 NM_001001935 NP_001001935 NM_001001937 NP_001001937 NM_001257334 NP_001244263 NM_001257335 NP_001244264 NM_004046 NP_004037
Function	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). {ECO:0000250}.
Subcellular Location	Mitochondrion inner membrane. Cell membrane; Peripheral membrane protein; Extracellular side. Note=Colocalizes with HRG on the cell surface of T-cells.
Tissue Specificity	Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. {ECO:0000269\|PubMed:8428659}.
Top Pathways	Alzheimer's disease, Huntington's disease, Oxidative phosphorylation

ATP5A (C-15) - sc-49162 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ATP5A (H-252) - sc-134728 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

TP5A (H-252): sc-134728. Western blot analysis of ATP5A expression in HeLa whole cell lysate.

ATP5A (N-18) - sc-49164 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ATP5A (N-18): sc-49164. Western blot analysis of ATP5A expression in Jurkat whole cell lysate.

ATP5A (51) - sc-136178 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Mouse
Clonality	Monoclonal

ATP5A (51): sc-136178. Western blot analysis of ATP5A expression in HeLa whole cell lysate.

Anti-ATP5A antibody - ab118482 from Abcam

Prices	$384.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ab118482 at 5&microg/ml staining ATP5A in Formalin-fixed, Paraffin-embedded Human breast tissue by immunohistochemistry.

Anti-ATP5A antibody - ab103431 from Abcam

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A antibody (ab103431) at 1/100 dilution + NCI-H460 cell lysate at 35 µg

Anti-ATP5A antibody - ab136082 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A antibody (ab136082) at 1/500 dilution + HT29 cell lysate at 30 µg

Anti-ATP5A antibody [EPR13030(B)] - ab176569 from Abcam

Prices	$397.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13030(B)

Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 at a 1/100 dilution (green). DAPI nuclear staining (blue).

Anti-ATP5A antibody [EPR13030(B)] (Alexa Fluor® 488) - ab196467 from Abcam

Prices	$431.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13030(B)

Overlay histogram showing HeLa cells stained with ab196467 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196467, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ATP5A antibody [EPR13030(B)] (Alexa Fluor® 647) - ab196198 from Abcam

Prices	$431.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13030(B)

Overlay histogram showing HeLa cells stained with ab196198 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196198, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ATP5A antibody [EPR13030(B)] (HRP) - ab195891 from Abcam

Prices	$425.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13030(B)

IHC image of ATP5A staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195891 at 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-ATP5A antibody [EPR13031] - ab178421 from Abcam

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13031

All lanes : Anti-ATP5A antibody [EPR13031] (ab178421) at 1/1000 dilutionLane 1 : HepG2 cell lysatesLane 2 : HeLa cell lysatesLane 3 : Human fetal liver lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa83-94) IHC-plus™ - LS-B11269 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-ATP5A1 / ATP Synthase Alpha Antibody (Lys239) - LS-C192116 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512, Biotin) - LS-C251987 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512, AP) - LS-C251986 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512, APC) - LS-C251988 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512, FITC) - LS-C251989 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512, Azide-free, HRP) - LS-C251990 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512, PE) - LS-C251991 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512) - LS-C329238 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa483-512) - LS-C156652 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

ATP5A1 Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-ATP5A1 / ATP Synthase Alpha Antibody - LS-C334361 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using ATP5A1 antibody.

Anti-ATP5A1 / ATP Synthase Alpha Antibody (aa201-250) IHC-plus™ - LS-B5741 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATP5A1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5741 concentration 5 ug/ml.

ATP5A Antibody - NBP2-15512 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.

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