Western blot analysis of PRAS40 phosphorylation in untreated (A,D), insulin treated (B,E) and insulin and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-PRAS40 (Thr 246): sc-32629 (A,B,C) and PRAS40 (H-216): sc-67042 (D,E,F).
Western blot analysis of PRAS40 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-PRAS40 (Thr 246): sc-32629 (A,B,C) and PRAS40 (H-216): sc-67042 (D,E,F).