ab85068 at 1/100 dilution staining ATPB in human brain by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Note cytoplasmic staining.
All lanes : Anti-ATPB antibody (ab85068) at 1/500 dilutionLane 1 : QGY-7703 cell lysateLane 2 : SMMC-7721 cell lysateLane 3 : PRF-5 cell lysate
ICC/IF image of ab85068 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85068, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.