Detection of Human ATRIP using ab17962 by Western Blot (2 x left images) and Immunoprecipitation (right image).Samples: Whole cell lysate (100 µg for WB; 400 µg for IP) from HeLa (A & B) and 293-T (A) cells. Antibodies: Affinity purified rabbit anti-ATRIP antibody (ab17962) used at 1/500 and 1/15000 dilution for WB and 3 µg/0.4 mg lysate for IP. ATRIP was also immunoprecipitated using a control antibody generated to recombinant human ATRIP:Right image - lane 1 ab17962; lane 2 control antibody; lane 3 normal IgG.Immunoprecipitated ATRIP was detected using the antibody to the recombinant protein. Detection: Chemiluminescence with exposure times of 5 minutes (2 x left) and 45 seconds (right image).