PTEN siRNA (h): sc-29459. Western blot analysis of PTEN expression in non-transfected control (A) and PTEN siRNA transfected (B) HeLa cells. Blot probed with PTEN (A2B1): sc-7974. Lamin A/C (H-110): sc-20681 used as specificity and loading control.
PTEN (A2B1): sc-7974. Western blot analysis of PTEN expression in non-transfected 293T: sc-117752 (A), mouse PTEN transfected 293T: sc-122834 (B) and HeLa (C) whole cell lysates.
PTEN (A2B1): sc-7974. Western blot analysis of PTEN expression in A-431 (A) and KNRK (B) whole cell lysates.
PTEN (A2B1): sc-7974. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
PTEN (A2B1): sc-7974. Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
PTEN HDR Plasmid (h): sc-400103-HDR. Western blot analysis of PTEN expression in non-transfected control (A) and puromycin treated, PTEN CRISPR/Cas9 KO Plasmid: sc-400103 plus PTEN HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with PTEN A2B1): sc-7974. β-Actin (C4): sc-47778 used as specificity and loading control.