Immunofluorescent analysis of HeLa cells (fixed in 4% paraformaldehyde at RT for 15 min) labeling ATRX with ab184228 at 1/500 dilution (Green). Hoechst 33342 staining (Blue).
All lanes : Anti-ATRX antibody [GT4212] - C-terminal (ab184228) at 1/500 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLysates/proteins at 50 µg per lane.
All lanes : Anti-ATRX antibody [GT4212] - C-terminal (ab184228) at 1/500 dilutionLane 1 : Neuro2A whole cell lysate/extractLane 2 : C8D30 whole cell lysate/extractLane 3 : BCL1 whole cell lysate/extractLysates/proteins at 30 µg per lane.