Anti-ATX2 antibody
| Name | Anti-ATX2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72263 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Chimpanzee, Monkey, Ape, Orangutan, Platypus |
| Antigen | Synthetic peptide from a region between residue 1262 and 1312 of human ATX2, NP_002964 |
| Description | Rabbit Polyclonal |
| Gene | ATXN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab72263 stained U-87 MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72263, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-ATX2 antibody (ab72263) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells at 50 µgLane 2 : Whole cell lysate from Hela cells at 15 µgLane 3 : Whole cell lysate from Hela cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of ATX2. Lane 1: ab72263 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72263 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.