PTEN (F-1): sc-393186. Western blot analysis of PTEN expression in non-transfected 293T: sc-117752 (A), mouse PTEN transfected 293T: sc-122834 (B), WI-38 (C) and MCF7 (D) whole cell lysates and human hippocampus tissue extract (E).
PTEN HDR Plasmid (h): sc-400103-HDR. Western blot analysis of PTEN expression in non-transfected control (A) and puromycin treated, PTEN CRISPR/Cas9 KO Plasmid: sc-400103 plus PTEN HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with PTEN (F-1): sc-393186. β-Actin (C4): sc-47778 used as specificity and loading control.