Anti-Aurora B antibody
| Name | Anti-Aurora B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2254 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F IHC-P IP WB |
| Species Reactivities | Mouse, Rat, Hamster, Human, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B |
| Description | Rabbit Polyclonal |
| Gene | AURKB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Aurora B staining in Human Lymphnode Hodgekins disease formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2254, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ICC/IF image of ab2254 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2254, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.
The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.
The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.
ab2254 staining human A431 (epithelial) cells by ICC/IF. The sample was fixed in paraformaldehyde and permeabilized by incubation with 0.1% Triton X100. 1% BSA was used as the blocking agent prior to a 1 hour incubation with the primary antibody, diluted 1/1000 with 1% BSA made up in PBS. An Alexa Fluor® 647 conjugated Donkey anti-Rabbit IgG (H+L) antibody was used as the secondary. Blocking and antibody incubation steps were carried out at room temperature.In this set of images, the tubulin is stained green, Aurora B in pink and DNA in blue.See Abreview
Immunofluorescence in human cells using Rabbit polyclonal to Aurora B (red), DAPI (blue) and CREST serum (binds to centromeres)(green).(a) HeLa cells - transition from interphase (left) through mitosis(b) RPE-1 cells - as in (a)(c) HeLa cells - interphase(d) RPE-1 cells - interphase
Rabbit polyclonal to Aurora B (ab2254) used to stain SW620 human tumour xenografts (in mouse).The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Aurora B antibody (ab2254) at 1 µg/mlLane 1 : HeLa Whole Cell LysateLane 2 : HeLa Nuclear LysateLane 3 : Jurkat Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
Product References
Selective disruption of aurora C kinase reveals distinct functions from aurora B - Selective disruption of aurora C kinase reveals distinct functions from aurora B
Balboula AZ, Schindler K. PLoS Genet. 2014 Feb 27;10(2):e1004194.
EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B. - EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B.
Banerjee B, Kestner CA, Stukenberg PT. J Cell Biol. 2014 Mar 17;204(6):947-63.
Chk2 prevents mitotic exit when the majority of kinetochores are unattached. - Chk2 prevents mitotic exit when the majority of kinetochores are unattached.
Petsalaki E, Zachos G. J Cell Biol. 2014 May 12;205(3):339-56.
Alisertib added to rituximab and vincristine is synthetic lethal and potentially - Alisertib added to rituximab and vincristine is synthetic lethal and potentially
Mahadevan D, Morales C, Cooke LS, Manziello A, Mount DW, Persky DO, Fisher RI, Miller TP, Qi W. PLoS One. 2014 Jun 3;9(6):e95184.
TAO1 kinase maintains chromosomal stability by facilitating proper congression of - TAO1 kinase maintains chromosomal stability by facilitating proper congression of
Shrestha RL, Tamura N, Fries A, Levin N, Clark J, Draviam VM. Open Biol. 2014 Jun;4(6):130108.
WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic - WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic
Vassilopoulos A, Tominaga Y, Kim HS, Lahusen T, Li B, Yu H, Gius D, Deng CX. Oncogene. 2014 Aug 4;0.
Aurora B prevents delayed DNA replication and premature mitotic exit by - Aurora B prevents delayed DNA replication and premature mitotic exit by
Trakala M, Fernandez-Miranda G, Perez de Castro I, Heeschen C, Malumbres M. Cell Cycle. 2013 Apr 1;12(7):1030-41.
Novel pyrimidine-2,4-diamine derivative suppresses the cell viability and spindle - Novel pyrimidine-2,4-diamine derivative suppresses the cell viability and spindle
Salmela AL, Pouwels J, Maki-Jouppila J, Kohonen P, Toivonen P, Kallio L, Kallio M. Carcinogenesis. 2013 Feb;34(2):436-45.
Stimulating myocardial regeneration with periostin Peptide in large mammals - Stimulating myocardial regeneration with periostin Peptide in large mammals
Ladage D, Yaniz-Galende E, Rapti K, Ishikawa K, Tilemann L, Shapiro S, Takewa Y, Muller-Ehmsen J, Schwarz M, Garcia MJ, Sanz J, Hajjar RJ, Kawase Y. PLoS One. 2013 May 20;8(5):e59656.
The Prp19 complex directly functions in mitotic spindle assembly. - The Prp19 complex directly functions in mitotic spindle assembly.
Hofmann JC, Tegha-Dunghu J, Drager S, Will CL, Luhrmann R, Gruss OJ. PLoS One. 2013 Sep 19;8(9):e74851.