AURKB

Gene Symbol AURKB
Entrez Gene 9212
Alt Symbol AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5, aurkb-sv1, aurkb-sv2
Species Human
Gene Type protein-coding
Description aurora kinase B
Other Description ARK-2|STK-1|aurora 1|aurora kinase B-Sv1|aurora kinase B-Sv2|aurora- and Ipl1-like midbody-associated protein 1|aurora-1|aurora-B|aurora-related kinase 2|aurora/IPL1-related kinase 2|protein phosphatase 1, regulatory subunit 48|serine/threonine kinase 12|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B
Swissprots C7G534 Q96GD4 Q96DV5 C7G535 J9JID1 Q9UQ46 D3DTR4 O60446 B4DNM4 C7G533 O14630 O95083
Accessions CAF04021 EAW90073 EAW90074 EAW90075 EAW90076 EAW90077 EAW90078 EAW90079 Q96GD4 AB011446 BAA82709 AB011450 BAA32136 AB519677 BAI23190 AB519678 BAI23191 AB519679 BAI23192 AF004022 AAB65786 AF008552 AAC12709 AF015254 AAC98891 AK297976 BAG60286 AK314889 BAG37403 AY677083 BC000442 AAH00442 BC009751 AAH09751 BC013300 AAH13300 BC080581 AAH80581 BE514591 BP225027 BT019534 AAV38341 XM_011524070 XP_011522372 XM_011524071 XP_011522373 XM_011524072 XP_011522374 XR_934117 XR_934118 NM_001256834 NP_001243763 NM_001284526 NP_001271455 NM_004217 NP_004208
Function Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission- competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex
Subcellular Location Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. Proper localization of the active, Thr-232- phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Colocalized with SIRT2 during cytokinesis with the midbody.
Tissue Specificity High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. {ECO:0000269|PubMed:9809983, ECO:0000269|PubMed:9858806}.

Aurora B/AIM1 Antibody - 3094 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Hela, NIH/3T3 and C6 cells, untreated or nocodazole-treated (50 ng/ml, 24 hrs), using Aurora B/AIM1 Antibody.

Phospho-Aurora B/AIM1(Thr232) Antibody - 3095 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ARK-2 (A-3) - sc-393357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARK-2 (A-3): sc-393357. Western blot analysis of ARK-2 expression in K-562 whole cell lysate.

ARK-2 (E-15) - sc-14326 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of ARK-2 expression in K-562 whole cell lysate (A) and mouse thymus tissue extract (B). Antibodies tested include ARK-2 (E-15): sc-14326 (A) and ARK-2 (E-20): sc-14327 (B).

ARK-2 (H-75) - sc-25426 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ARK-2 (H-75): sc-25426. Western blot analysis of human recombinant ARK-2.

ARK-2 (E-20) - sc-14327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of ARK-2 expression in K-562 whole cell lysate (A) and mouse thymus tissue extract (B). Antibodies tested include ARK-2 (E-15): sc-14326 (A) and ARK-2 (E-20): sc-14327 (B).

ARK-2 (13E8A7) - sc-65987 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARK-2 (13E8A7): sc-65987. Western blot analysis of human recombinant ARK-2.

Anti-Aurora B (phospho T232) antibody - ab115793 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Aurora B (phospho T232) with ab115793 at 5 ug/ml.  

Anti-Aurora B (phospho Y12) antibody - ab62466 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62466, at 1/50 dilution, staining Aurora B in human liver carcinoma tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

Anti-Aurora B antibody - ab2254 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of Aurora B staining in Human Lymphnode Hodgekins disease formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2254, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Aurora B antibody - ab70238 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.

Anti-Aurora B antibody - ab62702 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aurora B antibody (ab62702) at 1/500 dilutionLane 1 : Raw264.7 cell extracts treated with Hu (2nM, 24hours)Lane 2 : Raw264.7 cell extracts treated with Hu (2nM, 24hours) with immunising peptide

Anti-Aurora B antibody - ab37976 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Aurora B expression in formalin-fixed and paraffin-embedded human breast carcinoma tissue using 1/50 ab37976.

Anti-Aurora B antibody - ab61188 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aurora B antibody (ab61188) at 1/500 dilutionLane 1 : Cos7 cell extracts treated with Nocodazole (1 microgram/ml, 16 hours)Lane 2 : Cos7 cell extracts treated with Nocodazole (1 microgram/ml, 16 hours) with peptide

Anti-Aurora B antibody - ab189081 from Abcam

Prices $376.00
Sizes 50 µl
Host Sheep
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, labeling Aurora B with ab189081 at 10 ug/ml. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-Aurora B antibody [EP1009Y] - ab45145 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1009Y
Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/50000 dilution + HeLa cell lysate

Anti-Aurora B antibody [EP1009Y] (Alexa Fluor® 647) - ab197614 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1009Y
ab197614 staining Aurora B in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197614 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed A549 cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Aurora B antibody [mAbcam 10735] - ab10735 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 10735
Overlay histogram showing HeLa cells stained with ab10735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10735, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Aurora B antibody [mAbcam 10735] (FITC) - ab150283 from Abcam

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 10735

Anti-Aurora B antibody [mAbcam 3609] - ab3609 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 3609
All lanes : Anti-Aurora B antibody [mAbcam 3609] (ab3609) at 1 µg/mlLane 1 : Hek293 Whole Cell LysateLane 2 : Jurkat Whole Cell LysateLane 3 : HeLa Whole Cell LysateLane 4 : HeLa Whole Cell Lysate- Nocodozole StimulatedLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Absorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-AURKB / Aurora-B Antibody (phospho-Ser241) - LS-C3284 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-AURKB / Aurora-B Antibody (N-Terminus) - LS-C3857 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-AURKB / Aurora-B Antibody (aa1-19) - LS-C3858 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AURKB / Aurora-B Antibody (aa227-238) - LS-C3863 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-AURKB / Aurora-B Antibody (aa1-150) - LS-C35075 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Search more