Anti-Aurora B antibody
| Name | Anti-Aurora B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70238 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4) |
| Description | Rabbit Polyclonal |
| Gene | AURKB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
All lanes : Anti-Aurora B antibody (ab70238) at 1/10000 dilutionLane 1 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h) Lane 2 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h) Lane 3 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h) Lane 4 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)Lysates/proteins at 50 µg per lane.developed using the ECL technique
All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/mlLane 1 : HeLa whole cell lysate from an asynchronous cultureLane 2 : HeLa whole cell lysate treated with cycloheximide for 12 hoursLane 3 : HeLa whole cell lysatetreated with cycloheximide for 24 hoursLane 4 : HeLa whole cell lysate treated with cycloheximide for 36 hoursLane 5 : HeLa whole cell lysate treated with nocodazole for 20 hoursLysates/proteins at 100 µg per lane.
Product References
Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required - Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required
Martz MK, Grabocka E, Beeharry N, Yen TJ, Wedegaertner PB. Mol Biol Cell. 2013 Sep;24(18):2785-94.
Aurora B dynamics at centromeres create a diffusion-based phosphorylation - Aurora B dynamics at centromeres create a diffusion-based phosphorylation
Wang E, Ballister ER, Lampson MA. J Cell Biol. 2011 Aug 22;194(4):539-49.