All lanes : Anti-Aurora B antibody (ab62702) at 1/500 dilutionLane 1 : Raw264.7 cell extracts treated with Hu (2nM, 24hours)Lane 2 : Raw264.7 cell extracts treated with Hu (2nM, 24hours) with immunising peptide
ab62702 at 1/500 - 1/1000 dilution staining Aurora B in HeLa cells, treated with Forskolin (40nM, 30mins) by Immunofluorescence.
ab62702 at 1/50 - 1/100 dilution staining Aurora B in human liver carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.