All lanes : Anti-Axin 1 antibody (ab4157) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lysates/proteins at 20 µg per lane.SecondaryGoat IgG secondary antibody (ab28445) at 1/10000 dilution
ab4157 (3.8 µg/ml) staining Azin 1 in Human adrenal cortex by IHC-P (Immunohistochemistry of paraffin-embedded sections). Steamed antigen retrievalwith citrate buffer (pH 6) was performed. Image shows membranous and fibrous staining in and around the secretory cells of the zona fasciculata in the cortex.