Anti-Axin 2 antibody
| Name | Anti-Axin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab32197 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F ICC/IF ICC/IF WB FC |
| Species Reactivities | Mouse, Rat, Human, Chicken |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse Axin 2 |
| Description | Rabbit Polyclonal |
| Gene | AXIN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab32197 staining Axin2 in mouse colon sections. Secondary antibody conjugated to Alexa-fluor ® 488 (please see Abreview for further details)See Abreview
IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1:500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-Axin 2 antibody (ab32197) at 1 µg/mlLane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 2 : Colon (Mouse) Tissue Lysate Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate Lane 4 : LS147T (Colon cancer cells) (Human) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutionPerformed under reducing conditions.
Anti-Axin 2 antibody (ab32197) at 1 µg/ml + AXIN2 partial recombinant tagged protein at 0.1 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.
Product References
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A novel AXIN2 germline variant associated with attenuated FAP without signs of - A novel AXIN2 germline variant associated with attenuated FAP without signs of
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