Anti-Axin 2 antibody [EPR2005(2)]

• Supplier: Abcam
• Catalog: ab109307
• Prices: $390.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2005(2)
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Rat, Human, Pig
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Axin 2 aa 50-150
• Description: Rabbit Monoclonal
• Gene: AXIN2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)Lane 1 : SW480 cell lysateLane 2 : C6 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (purified)Lane 1 : SW430 cell lysateLane 2 : C6 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)Lane 1 : MCF7 cell lysates Lane 2 : SW480 cell lysates Lane 3 : PC3 cell lysates Lysates/proteins at 10 µg per lane.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Unpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview